Stem Cell-based Biosystems

Description:

Stem cell science has emerged as a novel discipline in biomedical research over the past decade. With advancements in this field, stem cells have demonstrated versatile applications ranging from basic research to clinical case studies. Recognizing the demand for knowledge in stem cell applications, this book aims to provide comprehensive information on stem cell technology and its application in biosystems. It covers fundamental culture methods, advanced stem cell-based biosystems, and clinical case studies of stem cell therapy. This book is intended for individuals with an interest in stem cell science, offering valuable insights into this rapidly evolving field.

Preface

In the past twenty years, there has been significant progress in stem cell science and technologies. With the deciphering of the biological properties of stem cells, scientists have been able to combined them with various technologies and utilize them for versatile applications in biomedical research. Recently, the emergence of stem cell therapy has led to an expectation of rapid growth in the stem cell-related market over the next decade, demonstrating the enormous economic value of related requirements. Simultaneously, these needs have also stimulated the development of the stem cell industry chain, which suggests that stem cell research will be expanded to an industry level. Therefore, stem cell-based biosystems have gained increasing attention.

This book presents the recent advances in stem cell-based biosystems, covering a broad spectrum of topics and state-of-the-art concepts in the field of stem cell application. The book is divided into three parts comprising ten chapters. The first part (Chapters 1–4) includes methods for isolating and culturing stem cells from different sources, recent technologies for regulating stem cell stemness through biomaterials, and common systems for culturing stem cells on a large scale. The second part (Chapters 5–8) discusses advanced technologies for biomimetic in vitro biosystems. In developing biomimetic in vitro biosystems, biomaterials play an indispensable role. Therefore, advanced technologies for two-dimensional (2D) and threedimensional (3D) biomaterials for fabricating stem cell-based biomimetic systems are introduced. Moreover, there are a large number of new drug or drug repurposing testing on living biomimetic 2D/3D biosystems in tissue engineering, regenerative medicine, and precision medicine field. Therefore, stem cell-based organ-on-chips for drug screening have also been introduced to enhance the prediction precision of testing effectiveness. Furthermore, biomaterials combined with nanotechnology as drug carriers for controlled release to regulate stem cell behavior is discussed for precision medicine applications in the second part.

The third part (Chapters 9–10) presents the evaluation of stem cell-based biosystems for applications from in vitro/ex vivo to clinical therapy approaches. Indepth discussions of the standard testing method of ex vivo and animal models for biomaterials and the guidelines and case studies of stem cell therapy for clinical applications are included. These novel and ongoing technologies provide new insights and possibilities for fabricating stem cell-based biosystems. Readers will receive a unique perspective on the challenges and emerging opportunities in the applications of stem cell-based biosystems.

Yi-Che Ethan Li Feng Chia University

I-Chi Lee National Tsing Hua University

Table of contents :

Cover
Title Page
Copyright Page
Preface
Table of Contents
1. Traditional Isolation and Culture Methods of Stem Cells
2. Biomaterial Effects on Isolation, Proliferation, and Regulation of Stem Cells
3. Scale-Up of Stem Cells
4. The Effects of Biomaterial Properties on the Behaviors of Stem Cells
5. The 2D Membrane Technology for Fabrication of 2D Stem Cell Culture System
6. The 3D Culture Technology for Fabrication of Stem Cell-based Biosystem
7. In vitro Biosystems—Stem Cell-based Organ on Chips
8. The Nanotechnology for Stem Cells
9. Biomaterials use in Ex vivo Testing and Animal Model
10. Guidance & Case Study for Stem Cell Therapy
Index